Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila
نویسندگان
چکیده
Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.
منابع مشابه
FIM, a Novel FTIR-Based Imaging Method for High Throughput Locomotion Analysis
We designed a novel imaging technique based on frustrated total internal reflection (FTIR) to obtain high resolution and high contrast movies. This FTIR-based Imaging Method (FIM) is suitable for a wide range of biological applications and a wide range of organisms. It operates at all wavelengths permitting the in vivo detection of fluorescent proteins. To demonstrate the benefits of FIM, we an...
متن کاملGenetically Expressed Cameleon in Drosophila melanogaster Is Used to Visualize Olfactory Information in Projection Neurons
Complex external stimuli such as odorants are believed to be internally represented in the brain by spatiotemporal activity patterns of extensive neuronal ensembles. These activity patterns can be recorded by optical imaging techniques. However, optical imaging with conventional fluorescence dyes usually does not allow for resolving the activity of biologically defined groups of neurons. Theref...
متن کاملEffect of Cognitive-Behavioral Group Therapy on Identity Crisis and Pregnancy of Pregnant Women Applying for IVF
Abstract Objective: The problem of infertility as a stressful event is associated with many psychological problems. The purpose of this study was to determine the effect of cognitive-behavioral group therapy on identity crisis and pregnancy probability of infertile women seeking IVF. Method: A quasi-experimental research with pre-test, post-test with control group was the statistical populati...
متن کاملFrontal behavioral inventory: diagnostic criteria for frontal lobe dementia.
OBJECTIVE To utilize the diagnostic criteria of frontal lobe dementia (FLD). METHODS We studied 12 patients with FLD diagnosed clinically, with radiological confirmation in 10 and autopsy confirmation in 2; sixteen patients with Alzheimer's disease matched for stage and severity to FLD and 11 patients with depressive dementia were used as control groups. A 24-item Frontal Behavioral Inventory...
متن کاملRab11 is required for maintenance of cell shape via βPS integrin mediated cell adhesion in Drosophila
In eukaryotes, vesicle trafficking is regulated by the small monomeric GTPases of the Rab protein family. Rab11, (a subfamily of the Ypt/Rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric Rab GTPases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. In an earlier communication, we have shown th...
متن کامل